Biomedical Applications of Vibrational Spectroscopy - Hyperspectral Imaging - Chemometrics

Introducing CytoSpec 64-bit (x64)
- CytoSpec 2.0 (Windows versions) -


Why Using CytoSpec 64-bit?
Compared to the standard version of CytoSpec 32-bit, CytoSpec 64-bit (x64) allows to handle much larger data files. While applications running under Windows 32-bit can use only less than 2 GB of memory, Windows 64-bit (x64) supports much larger amounts of RAM. This enables applications like CytoSpec to work with much larger data files and allows rapid data access by the 64-bit processor. The speed of the calculations is usually not affected.
What are the differences between 64-bit and 32-bit releases of CytoSpec (OS: Microsoft Windows)?
Except the larger address space which can be used by CytoSpec 64-bit, there are usually no functional differences between 32-bit and 64-bit editions of CytoSpec.
Exceptions: import routines for Thermo/Raman data files and for WITec *.mat files (Matlab formatted). Both import functions are available only for CytoSpec 64-bit.
Specification of computer configuration
  1. Processor: 64-bit CPU from Intel of AMD
  2. Operating system: Microsoft Windows 64-bit Systems: Windows 7/8/10
  3. Matlab Compiler Runtime (MCR) Environment R2014a 64-bit (The Mathworks). The MCR 2014a is available free of charge from The Mathworks website.
  4. CytoSpec 64-bit will be installed as a stand-alone application. A free software demo can be downloaded here (the license key must be ordered separately). Registered users can send an e-mail with their request to
  5. Memory: 4096 MB (4GB)) minimum. In order to use the advantages of the 64-bit architecture, the amount of installed memory (RAM) should be larger than 4 GB (8 GB of RAM, or more are recommended).
How to install the stand alone version of CytoSpec 64-bit (OS: Microsoft Windows)?
  1. Login with your Windows user account, download and unzip the CytoSpec 64-bit installation package.
  2. Download the Windows version of the Matlab Compiler Runtime (MCR) Environment R2014a 64-bit from the website of The Mathworks. Install the MCR2014a Windows 64-bit. Do not install other versions than MCR2014a 64-bit!
  3. Start CytoSpec.64bit.2.00.xx.setup.exe and follow the instructions of the installation routine
  4. Trial version users only: the license file 'cytospec.lic' is not included in the installation package. To register for a free, time-limited demo license please send an e-mail with your name and the name of the institution you are working for to the address
    The license key will be provided as an e-mail attachment. Copy the key file sent per e-mail into the DiaryDir folder of the CytoSpec installation (this is usually C:\Users\Public\Documents\Matlab, administrator rights are sometimes required).
  5. When setup is complete you can find a new program group and a CytoSpec shortcut on the desktop.
  6. Start the program by double clicking on the CytoSpec icon.
How to install CytoSpec pcode as a toolbox under Matlab (OS: Microsoft Windows)?
CytoSpec 64-bit can be also installed as a p-code toolbox under Matlab (OS: Microsoft Windows). This requires a full Matlab license. The following instructions refer to 64-bit CytoSpec pre-2.00.05 installations. Note that the CytoSpec p-code version will be discontinued with the availability of 64-bit stand alone CytoSpec v. 2.00.05.
  1. Create an additional directory e.g. '\ftir' in the existing toolbox directory structure of your Matlab installation. The complete path to the toolbox directory should be similar to
    'C:\program files\Matlab\R2014a\toolbox\matlab\ftir' (may vary!) with C:\program files\Matlab\R2014a equaling the variable 'matlabroot'. You can check this variable by typing
    >> matlabroot
    at the Matlab command prompt. Note that the directory 'C:\program files\Matlab\R2014a\toolbox\matlab' should contain additional subdirectories such as \winfun oder \timefun.
  2. Unpack now the zip archive '' and copy all files into the new subdirectory ...\ftir ('2.00.xx' denotes the pcode toolbox version number).
  3. Start Matlab and enter at the command prompt
    >> edit pathdef
  4. The editor will now load the file 'pathdef.m'. After the comment line
    %%% BEGIN ENTRIES %%%
    add a new line with the complete path to the CytoSpec toolbox
  5. Save pathdef.m then restart Matlab or type
    >> rehash toolbox
    at the Matlab command prompt.
  6. To run CytoSpec call the file 'ftir.p' by typing
    >> ftir
    at the Matlab command prompt.
  7. Function Synthon imaging: this function requires the files 'AnnEval.exe' and 'Neuro.dll ' being copied to the location C:\Program files (x86)\CytoSpec\CytoSpec
  8. Do not forget to copy your personal license key 'cytospec.lic' into the CytoSpec p-code toolbox directory ...\ftir!
Useful Links:
  1. CytoSpec for LINUX CytoSpec for LINUX has been released
  2. What are the benefits of Microsoft Windows x64 Editions
  3. Link to The Mathworks:
  4. Link to the download site of the Matlab Compiler Runtime (The Mathworks).
  5. What are the benefits of 64-bit MATLAB versus 32-bit MATLAB?
  6. What is Matlab pcode? What is the difference between MATLAB code and p-code in MATLAB?


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