CytoSpec - an APPLICATION FOR HYPERSPECTRAL IMAGING


 

File Pulldown Menu

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Imaging

Chemical Imaging
Frequency Maps
3D-Deconvolution
HCA Imaging
PCA Imaging
Synthon Imaging (ANN)
ANN Imaging

Preprocessing

Calculation of Derivative Spectra
Normalization (Vector, Offset)
Cut
Interpolate
Smooth
ABS <--> TR Conversion
Subtraction
Dispersion Correction
Quality test
Baseline Correction
Water Vapor Compensation
Noise Correction
Batch Preprocessing

Multivariate Statistics

Hierarchical Cluster Analysis
Principal Component Analysis
k-Means Clustering
Fuzzy C-Means Clustering

Tools

Display Options
Display Spectra
Set Display limits
Grid On/Off
Set Colors
Capture
Export Maps
Map Statistics
Display Large Maps
Define ROI
Display Colorbar
Swap Data Blocks
Rotate
Flip

File Information

Show History
Show Instrument Parameters
Show measurement Parameters
Show Additional Parameters
Edit Parameters

 

PULL DOWN MENU "FILE INFORMATION"

 

File Info Menu
 

SHOW DATA BLOCK HISTORY


 
    Show data block history: The history of data manipulations is stored within the spectral multifile. In order to view this information select 'File info --> show history' and select the data block of interest.
     
    The information includes:
     
      1.  data source, time and data of import (block of original data).
      2.  time and date of data block creation (data block of preprocessed, derivative, and 3D -FSD spectra)
      3.  parameters of preprocessing (derivatives, cut, smooth, interpolate, baseline correction, quality test, etc.)
       
      Additional information is available from the log-file.
       
show manipulations

 

SHOW PARAMETERS

 
    Displays parameter of the following data parameter blocks:
       
      1.  Instrument parameters
      2.  Measurement parameters
      3.  Additional data acquisition parameters
     
    Detailed information on the data parameters is available in the following chapter of this help manual. Additionally, the data parameters can be modified using the function Edit Parameters of the 'File Info' pull down menu.

EDIT PARAMETERS

 
 edit parameters
 
 
      Instrument parameters:       Measurement parameters:
       
INS - instrument type LWN - starting wavenumber
MAN - instrument manufacturer UWN - ending wavenumber
SRC - source setting WVS - wavenumber step
BSP - beamsplitter setting NOD - number of spectral data points
FIL - type of optical filter DAT - date of measurement
DET - type of detector TIM - time of measurement
VEL - scanner velocity PHC - phase correction mode
LAS - laser wavenumber PHR - phase resolution
AQM - acquisition mode PIP - phase interferogram points
SNG - signal gain ZFF - zero filling factor
    RES - nominal resolution
      Additional data acquisition parameters: APO - apodization function
    APT - aperture size (mkm)
TOS - type of spectra STX - step in x dimension (µm)
NOS - number of scans STY - step in y dimension (µm)
XDI - number of pixel in x-direction    
YDI - number of pixel in y-direction    
SZX - area size in x dimension (µm)    
SZY - area size in y dimension (µm)    
SAM - sample name    
SAF - sample form    
OPN - operator name    
AS1 - additional sample information 1    
AS2 - additional sample information 2    
AS3 - additional sample information 3    


 
    Parameters highlighted by red color should be carefully modified since these parameters are used by the software. Modification of these parameters may cause malfunctioning.
     
    Changes of the parameters highlighted by red color are tracked and checked when leaving the 'edit parameter' window. In case of errors or data inconsistencies an error messages will be displayed. The CytoSpec program also makes suggestions of how to change the faulty parameters (see command window below).
     
 wrong parameters - error message
 
 
 command line window: suggestion for changing parameters

 

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