What is CytoSpec?
The Multivariate Imaging Software package CytoSpec represents a novel approach for creating pseudo-color displays of spectral imaging data via statistical methods that utilize the entire spectral information. It is laid out and optimized to work on 3-dimensional spectral data sets from imaging or mapping infrared or Raman experiments. Native data formats from from various instrument manufacturers can be analyzed.
What is new in CytoSpec 1.4.02?
- A VCA (vertex component analysis) imaging routine
- Fuzzy C-means (FCM) clustering routine with enhanced functionality: A new option "composite image" was added. With CytoSpec 1.4.02 FCM can be carried out on the basis of several interspectral distance measures: Euclidean, standardized Euclidean and D-Values
- Modifications of the k-means cluster (KMC) imaging routine: KMC imaging allows to choose between several distance options: Euclidean, standardized Euclidean and D-Values. An option for modifying the learning rate was also added.
- New import filter for NT-MDT Raman data (ASCII only)
- Added a new import filter for Kaiser-Raman Holospec data (ASCII only)
- WITec import filter updated
- Added a new import filter for AFM and AFMIR data (Alex Dazzi/Universite Paris Sud)
- Agglomerative hierarchical cluster imaging (HCA): dramatic speed improvements in distance matrix calculation. Many thanks to Milos Miljkovic (Max Diem lab at Northeastern University) which provided the original source code. Speed advantage by a factor of 20 and more! (speed mode only)
- PCA imaging function re-designed. Permits now also "basic" PCA imaging
- Function "rotate" with a new option for rotating by an arbitrary angle
- Pull-down menus re-arranged. With version 1.4.02 CytoSpec has now the a pulldown menu "Univariate Imaging" (frequency, chemical imaging) and "Multivariate Imaging" containing ANN/Cluster and VCA imaging functions
- 3D-FSD is now a part of the preprocessing pulldown menu
- Inverted colormaps added: i-jet, i-hsv and i-hot for displaying maps from derivative data
- Perkin-Elmer and Bruker-OPUS import routines will load also incomplete maps (will display an error message)
- Pre-processing function "subtract": subtraction of hyperspectral data sets by internal or external vibrational spectra possible
- Macro-programming for automated pre-processing added
- Many code improvements & bug fixes
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